Elucidating the mechanism of cellular uptake and removal of protein

Differences in cytotoxicity among these three were not significant (Fig.

The intracellular concentrations of gold after incubation for 4h with the P-GNPs were below the detection limit of inductively coupled plasma atomic emission spectrometer (ICP-AES).

), which corresponded to the transverse longitudinal plasmon resonance of the elongated tips.

The GNRs were observed to have two SPR peaks at 510nm and 800nm, which were also related to the transverse and longitudinal modes (Fig.

Gold nanomaterials have attracted considerable interest as vehicles for intracellular drug delivery.

In our study, we synthesized three different shapes of methylpolyethylene glycol coated-anisotropic gold nanoparticles: stars, rods, and triangles.

The possible mechanisms of cellular uptake for the three types of gold nanoparticles were examined, and it was found that different shapes tended to use the various endocytosis pathways in different proportions.

elucidating the mechanism of cellular uptake and removal of protein-17

A greater understanding of the shape effect on GNP-cell interactions would aid the development of effective tools for drug delivery.However, there have been few studies on cellular uptake of GNPs with different shapes and most of the attention has been given to spherical nanoparticles.It has been shown that the cellular uptake of rod-like GNPs by He La cells is less efficient than that of spherical ones.At 8h and 24h, shape and time-dependent cellular uptake was observed (Fig. After incubation for 24h, gold concentrations in the cells incubated with P-GNSs, P-GNRs, and P-GNTs were 0.154±0.010pg/cell, 0.814±0.001pg/cell, and 1.333±0.038pg/cell, respectively.When the data were converted to percentage uptake from the total added gold the results were 0.38%, 2.04%, and 3.33%.